DUN1
Gene Ontology Biological Process
Gene Ontology Molecular Function
PLB2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Dosage Lethality
A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.
Publication
Functional analysis with a barcoder yeast gene overexpression system.
Systematic analysis of gene overexpression phenotypes provides an insight into gene function, enzyme targets, and biological pathways. Here, we describe a novel functional genomics platform that enables a highly parallel and systematic assessment of overexpression phenotypes in pooled cultures. First, we constructed a genome-level collection of ~5100 yeast barcoder strains, each of which carries a unique barcode, enabling pooled fitness ... [more]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- conditional SDL hits in 0.0001% MMS (15 Generation of treatment)
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
PLB2 DUN1 | Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores. | High | 2.0274 | BioGRID | 322439 |
Curated By
- BioGRID