BAIT

POL1

polA, swi7, SPAC3H5.06c
DNA polymerase alpha catalytic subunit
Schizosaccharomyces pombe (972h)
PREY

CRB2

rhp9, SPBC342.05
DNA repair protein Rad9 homolog, Rhp9
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Regulation of checkpoint kinases through dynamic interaction with Crb2.

Mochida S, Esashi F, Aono N, Tamai K, O'Connell MJ, Yanagida M

ATR/Rad3-like kinases promote the DNA damage checkpoint through regulating Chk1 that restrains the activation of cyclin-dependent kinases. In fission yeast, Crb2, a BRCT-domain protein that is similar to vertebrate 53BP1, plays a crucial role in establishing this checkpoint. We report here that Crb2 regulates DNA damage checkpoint through temporal and dynamic interactions with Rad3, Chk1 and replication factor Cut5. The ... [more]

EMBO J. Jan. 28, 2004; 23(2);418-28 [Pubmed: 14739927]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: mitotic cell cycle (APO:0000072)

Additional Notes

  • mutation of crb2 inhibits the checkpoint arrest induced by polymerase mutations causing an increase in the frequency of abnormal mitotic cells

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CRB2 POL1
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
247057
POL1 CRB2
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
795461
CRB2 POL1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
247064

Curated By

  • BioGRID