CUBN
Gene Ontology Biological Process
- cobalamin metabolic process [TAS]
- cobalamin transport [TAS]
- lipoprotein metabolic process [TAS]
- receptor-mediated endocytosis [NAS]
- small molecule metabolic process [TAS]
- steroid metabolic process [TAS]
- tissue homeostasis [NAS]
- vitamin D metabolic process [TAS]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
LRP1
Gene Ontology Biological Process
- aorta morphogenesis [ISS]
- apoptotic cell clearance [ISS]
- beta-amyloid clearance [TAS]
- lipoprotein transport [NAS]
- negative regulation of Wnt signaling pathway [ISS]
- negative regulation of platelet-derived growth factor receptor-beta signaling pathway [ISS]
- negative regulation of smooth muscle cell migration [ISS]
- phototransduction, visible light [TAS]
- positive regulation of cholesterol efflux [ISS]
- positive regulation of lipid transport [ISS]
- regulation of actin cytoskeleton organization [ISS]
- regulation of cholesterol transport [ISS]
- regulation of phospholipase A2 activity [ISS]
- retinoid metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Identification of the ligands of protein interaction domains through a functional approach.
The identification of protein-protein interaction networks has often given important information about the functions of specific proteins and on the cross-talk among metabolic and regulatory pathways. The availability of entire genome sequences has rendered feasible the systematic screening of collections of proteins, often of unknown function, aimed to find the cognate ligands. Once identified by genetic and/or biochemical approaches, the ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 6.
Curated By
- BioGRID