An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Tubulin is an inherent component of mitochondrial membranes that interacts with the voltage-dependent anion channel.

Carre M, Andre N, Carles G, Borghi H, Brichese L, Briand C, Braguer D

We have previously reported that anti-tubulin agents induce the release of cytochrome c from isolated mitochondria. In this study, we show that tubulin is present in mitochondria isolated from different human cancerous and non-cancerous cell lines. The absence of polymerized microtubules and cytosolic proteins was checked to ensure that this tubulin is an inherent component of the mitochondria. In addition, ... [more]

J. Biol. Chem. Sep. 13, 2002; 277(37);33664-9 [Pubmed: 12087096]

Throughput

Low Throughput

Additional Notes

figure 6.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TUBA1B VDAC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Carre M (2002)	Low	-	BioGRID	808695

Curated By

BioGRID

Interaction Summary

VDAC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein kinase binding [IPI]voltage-gated anion channel activity [ISS, TAS]

Gene Ontology Cellular Component

TUBA1B

Gene Ontology Biological Process

Gene Ontology Molecular Function

double-stranded RNA binding [IDA]protein binding [IPI]structural molecule activity [TAS]ubiquitin protein ligase binding [IPI]