STUB1
Gene Ontology Biological Process
- cellular response to misfolded protein [IDA]
- misfolded or incompletely synthesized protein catabolic process [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- positive regulation of chaperone-mediated protein complex assembly [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein ubiquitination [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein K63-linked ubiquitination [IDA]
- protein autoubiquitination [IDA]
- protein maturation [TAS]
- protein polyubiquitination [IDA, IMP]
- regulation of glucocorticoid metabolic process [IDA]
- transforming growth factor beta receptor signaling pathway [TAS]
- ubiquitin-dependent SMAD protein catabolic process [IDA]
- ubiquitin-dependent protein catabolic process [IMP]
Gene Ontology Molecular Function- G-protein coupled receptor binding [IPI]
- Hsp70 protein binding [IDA]
- Hsp90 protein binding [IDA]
- SMAD binding [IDA]
- TPR domain binding [IDA]
- enzyme binding [IPI]
- kinase binding [IPI]
- misfolded protein binding [IDA]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein homodimerization activity [ISS]
- ubiquitin protein ligase activity [IDA]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA, IMP, TAS]
- ubiquitin-ubiquitin ligase activity [ISS]
- G-protein coupled receptor binding [IPI]
- Hsp70 protein binding [IDA]
- Hsp90 protein binding [IDA]
- SMAD binding [IDA]
- TPR domain binding [IDA]
- enzyme binding [IPI]
- kinase binding [IPI]
- misfolded protein binding [IDA]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein homodimerization activity [ISS]
- ubiquitin protein ligase activity [IDA]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA, IMP, TAS]
- ubiquitin-ubiquitin ligase activity [ISS]
Gene Ontology Cellular Component
NOS1
Gene Ontology Biological Process
- arginine catabolic process [IC]
- blood coagulation [TAS]
- cellular response to growth factor stimulus [ISS]
- exogenous drug catabolic process [ISS]
- interaction with host [TAS]
- multicellular organismal response to stress [IMP]
- myoblast fusion [TAS]
- negative regulation of blood pressure [IBA]
- negative regulation of calcium ion transport [ISS]
- negative regulation of calcium ion transport into cytosol [TAS]
- negative regulation of hydrolase activity [ISS]
- negative regulation of potassium ion transport [ISS]
- negative regulation of serotonin uptake [ISS]
- neurotransmitter biosynthetic process [TAS]
- nitric oxide biosynthetic process [IBA, ISS]
- nitric oxide mediated signal transduction [IBA]
- peptidyl-cysteine S-nitrosylation [ISS]
- phagosome maturation [TAS]
- positive regulation of guanylate cyclase activity [IBA]
- positive regulation of histone acetylation [ISS]
- positive regulation of sodium ion transmembrane transport [ISS]
- positive regulation of the force of heart contraction [ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of transcription, DNA-templated [ISS]
- positive regulation of vasodilation [IDA, IMP]
- regulation of cardiac muscle contraction [TAS]
- regulation of sodium ion transport [ISS]
- response to heat [IDA]
- response to hypoxia [IEP]
- striated muscle contraction [ISS]
Gene Ontology Molecular Function- FMN binding [ISS]
- NADP binding [ISS]
- NADPH-hemoprotein reductase activity [IBA]
- arginine binding [TAS]
- cadmium ion binding [ISS]
- flavin adenine dinucleotide binding [ISS]
- heme binding [ISS]
- ion channel binding [ISS]
- nitric-oxide synthase activity [IBA, ISS]
- oxidoreductase activity [IBA]
- protein binding [IPI]
- scaffold protein binding [ISS]
- sodium channel regulator activity [ISS]
- tetrahydrobiopterin binding [NAS]
- FMN binding [ISS]
- NADP binding [ISS]
- NADPH-hemoprotein reductase activity [IBA]
- arginine binding [TAS]
- cadmium ion binding [ISS]
- flavin adenine dinucleotide binding [ISS]
- heme binding [ISS]
- ion channel binding [ISS]
- nitric-oxide synthase activity [IBA, ISS]
- oxidoreductase activity [IBA]
- protein binding [IPI]
- scaffold protein binding [ISS]
- sodium channel regulator activity [ISS]
- tetrahydrobiopterin binding [NAS]
Gene Ontology Cellular Component
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Ubiquitination of neuronal nitric-oxide synthase in the calmodulin-binding site triggers proteasomal degradation of the protein.
Nitric-oxide synthase, a cytochrome P450-like hemoprotein enzyme, catalyzes the synthesis of nitric oxide, a critical signaling molecule in a variety of physiological processes. Our laboratory has discovered that certain drugs suicide-inactivate neuronal nitric-oxide synthase (nNOS) and lead to the preferential ubiquitination of the inactivated nNOS by an Hsp70- and CHIP (C terminus of Hsc70-interacting protein)-dependent process. To further understand the ... [more]
Throughput
- Low Throughput
Additional Notes
- E2 (UbcH5a)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NOS1 STUB1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| STUB1 NOS1 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 593002 | |
| STUB1 NOS1 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 2972512 | |
| STUB1 NOS1 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 472072 |
Curated By
- BioGRID