BAIT

PER3

GIG13, RP3-467L1.3
period circadian clock 3
GO Process (1)
GO Function (1)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens
PREY

ATM

AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1
ATM serine/threonine kinase
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Per3, a circadian gene, is required for Chk2 activation in human cells.

Im JS, Jung BH, Kim SE, Lee KH, Lee JK

PER3 is a member of the PERIOD genes, but does not play essential roles in the circadian clock. Depletion of Per3 by siRNA almost completely abolished activation of checkpoint kinase 2 (Chk2) after inducing DNA damage in human cells. In addition, Per3 physically interacted with ATM and Chk2. Per3 overexpression induced Chk2 activation in the absence of exogenous DNA damage, ... [more]

FEBS Lett. Dec. 01, 2010; 584(23);4731-4 [Pubmed: 21070773]

Throughput

  • Low Throughput

Curated By

  • BioGRID