BAIT

NAP1

histone chaperone NAP1, L000001232, YKR048C
Histone chaperone; involved in histone exchange by removing and replacing histone H2A-H2B dimers or histone variant dimers from assembled nucleosomes; involved in the transport of H2A and H2B histones to the nucleus; required for the regulation of microtubule dynamics during mitosis; interacts with mitotic cyclin Clb2p; controls bud morphogenesis; phosphorylated by CK2; protein abundance increases in response to DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

ELP2

KTI3, TOT2, Elongator subunit ELP2, YGR200C
Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
GO Process (3)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Histone chaperones Nap1 and Vps75 regulate histone acetylation during transcription elongation.

Xue YM, Kowalska AK, Grabowska K, Przybyt K, Cichewicz MA, Del Rosario BC, Pemberton LF

Histone chaperones function in chromatin assembly and disassembly suggesting they have important regulatory roles in transcription elongation. The Saccharomyces cerevisiae proteins, Nap1 and Vps75, are structurally related, evolutionarily conserved histone chaperones. We showed that Nap1 genetically interacts with several transcription elongation factors, and both Nap1 and Vps75 interact with the RNA polymerase II kinase, CTK1. Loss of NAP1 or VPS75 ... [more]

Mol. Cell. Biol. Feb. 11, 2013; 0(0); [Pubmed: 23401858]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: budding (APO:0000024)

Additional Notes

  • double mutant exhibits greatly increased frequency of multibudded cells

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NAP1 ELP2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.116BioGRID
2431457
ELP2 NAP1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

High-BioGRID
483339

Curated By

  • BioGRID