Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Calmodulin antagonizes a calcium-activated SCF ubiquitin E3 ligase subunit, FBXL2, to regulate surfactant homeostasis.

Chen BB, Coon TA, Glasser JR, Mallampalli RK

Calmodulin is a universal calcium-sensing protein that has pleiotropic effects. Here we show that calmodulin inhibits a new SCF (Skp1-Cullin-F-box) E3 ligase component, FBXL2. During Pseudomonas aeruginosa infection, SCF (FBXL2) targets the key enzyme, CCTÎ±, for its monoubiquitination and degradation, thereby reducing synthesis of the indispensable membrane and surfactant component, phosphatidylcholine. P. aeruginosa triggers calcium influx and calcium-dependent activation of ... [more]

Mol. Cell. Biol. May. 01, 2011; 31(9);1905-20 [Pubmed: 21343341]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FBXL2 CALM1	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Chen BB (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FBXL2

Gene Ontology Biological Process

Gene Ontology Molecular Function

phosphatidylinositol 3-kinase regulatory subunit binding [ISO]protein binding [IPI]protein phosphatase binding [ISO]ubiquitin-protein transferase activity [IMP, ISO]

Gene Ontology Cellular Component

CALM1