An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A proteomic investigation of ligand-dependent HSP90 complexes reveals CHORDC1 as a novel ADP-dependent HSP90-interacting protein.

Gano JJ, Simon JA

Structural studies of the chaperone HSP90 have revealed that nucleotide and drug ligands induce several distinct conformational states; however, little is known how these conformations affect interactions with co-chaperones and client proteins. Here we use tandem affinity purification and LC-MS/MS to investigate the proteome-wide effects of ATP, ADP, and geldanamycin on the constituents of the human HSP90 interactome. We identified ... [more]

Mol. Cell Proteomics Feb. 01, 2010; 9(2);255-70 [Pubmed: 19875381]

Throughput

High Throughput

Ontology Terms

cell line: hek-293 cell (BTO:0000007) [geldanamycin (CHEBI:5292)]

Additional Notes

interaction increased in the presence of geldanamycin
proteins pulled down using a TAP-tagged N- or C-terminal fragment of HSP90AA1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HSP90AA1 TOMM34	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Skarra DV (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

HSP90AA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [TAS]ATPase activity [IDA]MHC class II protein complex binding [IDA]TPR domain binding [IDA, TAS]identical protein binding [IPI]nitric-oxide synthase regulator activity [IDA]nucleotide binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein homodimerization activity [TAS]

Gene Ontology Cellular Component

TOMM34