BAIT

CDC-6

CELE_C43E11.10, C43E11.10
cdc-6 encodes a homolog of an origin complex component (CDC6) which in yeast controls the start of DNA replication, and also has a distant paralog (Y39A1A.12) within the C. elegans genome; CDC-6 is superficially dispensable for embryonic viability, perhaps because of redundancy with Y39A1A.12.
Caenorhabditis elegans
PREY

IMA-3

CELE_F32E10.4, F32E10.4
ima-3 encodes one of three C. elegans importin alpha nuclear transport factors and the importin alpha that is most similar to the alpha3-subtype; ima-3 activity is required throughout development: during oogenesis, ima-3 is essential for progression past pachytene of meiotic prophase I and for proper organization of the nuclear pore complex (NPC) as well as association of P granules with the NPC; ima-3 is also required for normal embryonic, larval, and germline development; in vitro, IMA-3 can interact with human importin beta1, suggesting that it functions in a complex with C. elegans importin betas in vivo; IMA-3 is expressed in males and hermaphrodites, in both the germline and in somatic tissue; in the germline, IMA-3 is seen in the common cytoplasm of the rachis and in association with the nuclear envelope of germline nuclei and the residual body of developing spermatids.
Caenorhabditis elegans

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A protein domain-based interactome network for C. elegans early embryogenesis.

Boxem M, Maliga Z, Klitgord N, Li N, Lemmens I, Mana M, de Lichtervelde L, Mul JD, van de Peut D, Devos M, Simonis N, Yildirim MA, Cokol M, Kao HL, de Smet AS, Wang H, Schlaitz AL, Hao T, Milstein S, Fan C, Tipsword M, Drew K, Galli M, Rhrissorrakrai K, Drechsel D, Koller D, Roth FP, Iakoucheva LM, Dunker AK, Bonneau R, Gunsalus KC, Hill DE, Piano F, Tavernier J, van den Heuvel S, Hyman AA, Vidal M

Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 ... [more]

Cell Aug. 08, 2008; 134(3);534-45 [Pubmed: 18692475]

Throughput

  • High Throughput

Additional Notes

  • Corrected dataset: Erratum in Cell. 2012 Dec 21;151(7):1633.

Curated By

  • BioGRID