Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

CatSperbeta, a novel transmembrane protein in the CatSper channel complex.

Liu J, Xia J, Cho KH, Clapham DE, Ren D

Four CatSper ion channel subunit genes (CatSpers 1-4) are required for sperm cell hyperactivation and male fertility. The four proteins assemble (presumably as a tetramer) to form a sperm-specific, alkalinization-activated Ca(2+)-selective channel. We set out to identify proteins associating with CatSper that might help explain its unique role in spermatozoa. Using a transgenic approach, a CatSper1 complex was purified from ... [more]

J. Biol. Chem. Jun. 29, 2007; 282(26);18945-52 [Pubmed: 17478420]

Throughput

  • Low Throughput

Curated By

  • BioGRID