BAIT
RAD51
MUT5, recombinase RAD51, L000001571, YER095W
Strand exchange protein; forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
GO Process (7)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
CDC42
Rho family GTPase CDC42, L000000276, YLR229C
Small rho-like GTPase; essential for establishment and maintenance of cell polarity; plays a role late in cell fusion via activation of key cell fusion regulator Fus2p; mutants have defects in the organization of actin and septins
GO Process (13)
GO Function (1)
GO Component (8)
Gene Ontology Biological Process
- budding cell apical bud growth [IMP]
- budding cell isotropic bud growth [IMP]
- conjugation with cellular fusion [IMP]
- establishment of cell polarity [IMP]
- invasive growth in response to glucose limitation [IMP]
- pheromone-dependent signal transduction involved in conjugation with cellular fusion [IGI, IMP]
- positive regulation of exocytosis [IGI, IMP, IPI]
- positive regulation of pseudohyphal growth [IMP]
- regulation of exit from mitosis [IMP]
- regulation of exocyst localization [IMP]
- regulation of initiation of mating projection growth [IMP]
- regulation of vacuole fusion, non-autophagic [IMP]
- septin ring organization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Saccharomyces cerevisiae Genetics Predicts Candidate Therapeutic Genetic Interactions at the Mammalian Replication Fork.
The concept of synthetic lethality has gained popularity as a rational guide for predicting chemotherapeutic targets based on negative genetic interactions between tumor-specific somatic mutations and a second-site target gene. One hallmark of most cancers that can be exploited by chemotherapies is chromosome instability (CIN). Because chromosome replication, maintenance, and segregation represent conserved and cell-essential processes, they can be modeled ... [more]
G3 (Bethesda) Feb. 01, 2013; 3(2);273-82 [Pubmed: 23390603]
Quantitative Score
- 0.025489123 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- SGA analysis for synthetic lethal interactions between mutations whose human orthologs are found to be mutated in cancers, and the deletion mutant collection, where the interaction probability P < 0.05
Curated By
- BioGRID