Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Development and application of a DNA microarray-based yeast two-hybrid system.

Suter B, Fontaine JF, Yildirimman R, Rasko T, Schaefer MH, Rasche A, Porras P, Vazquez-Alvarez BM, Russ J, Rau K, Foulle R, Zenkner M, Saar K, Herwig R, Andrade-Navarro MA, Wanker EE

The yeast two-hybrid (Y2H) system is the most widely applied methodology for systematic protein-protein interaction (PPI) screening and the generation of comprehensive interaction networks. We developed a novel Y2H interaction screening procedure using DNA microarrays for high-throughput quantitative PPI detection. Applying a global pooling and selection scheme to a large collection of human open reading frames, proof-of-principle Y2H interaction screens ... [more]

Nucleic Acids Res. Feb. 01, 2013; 41(3);1496-507 [Pubmed: 23275563]

Throughput

High Throughput

Additional Notes

expanded Ataxin (ATXN1-Q79)
wild-type Ataxin (ATXN1-Q32)

Curated By

BioGRID

Interaction Summary

ATXN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]poly(G) binding [IDA]poly(U) RNA binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]protein self-association [IDA]

Gene Ontology Cellular Component

MLST8