Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Dynamic protein-protein interaction wiring of the human spliceosome.

Hegele A, Kamburov A, Grossmann A, Sourlis C, Wowro S, Weimann M, Will CL, Pena V, Luehrmann R, Stelzl U

More than 200 proteins copurify with spliceosomes, the compositionally dynamic RNPs catalyzing pre-mRNA splicing. To better understand protein - protein interactions governing splicing, we systematically investigated interactions between human spliceosomal proteins. A comprehensive Y2H interaction matrix screen generated a protein interaction map comprising 632 interactions between 196 proteins. Among these, 242 interactions were found between spliceosomal core proteins and largely ... [more]

Mol. Cell Feb. 24, 2012; 45(4);567-80 [Pubmed: 22365833]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RBM10 RBM10	Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.	Hegele A (2012)	Low	-	BioGRID	-
RBM10 RBM10	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Wang XW (2023)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RBM10

Gene Ontology Molecular Function

identical protein binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]protein complex binding [IDA]

Gene Ontology Cellular Component