An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Publication

Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition.

Taipale M, Krykbaeva I, Koeva M, Kayatekin C, Westover KD, Karras GI, Lindquist S

HSP90 is a molecular chaperone that associates with numerous substrate proteins called clients. It plays many important roles in human biology and medicine, but determinants of client recognition by HSP90 have remained frustratingly elusive. We systematically and quantitatively surveyed most human kinases, transcription factors, and E3 ligases for interaction with HSP90 and its cochaperone CDC37. Unexpectedly, many more kinases than ... [more]

Cell Aug. 31, 2012; 150(5);987-1001 [Pubmed: 22939624]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

HSP90AA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [TAS]ATPase activity [IDA]MHC class II protein complex binding [IDA]TPR domain binding [IDA, TAS]identical protein binding [IPI]nitric-oxide synthase regulator activity [IDA]nucleotide binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein homodimerization activity [TAS]

Gene Ontology Cellular Component

MAP3K12