BAIT
HSP90AA1
EL52, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP-2, LAP2
heat shock protein 90kDa alpha (cytosolic), class A member 1
GO Process (16)
GO Function (10)
GO Component (10)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- axon guidance [TAS]
- chaperone-mediated protein complex assembly [IDA]
- innate immune response [TAS]
- mitochondrial transport [TAS]
- mitotic cell cycle [TAS]
- nitric oxide metabolic process [TAS]
- positive regulation of nitric oxide biosynthetic process [ISS]
- protein import into mitochondrial outer membrane [IDA]
- protein refolding [TAS]
- regulation of nitric-oxide synthase activity [TAS]
- response to unfolded protein [NAS]
- signal transduction [NAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NR1H3
LXR-a, LXRA, RLD-1
nuclear receptor subfamily 1, group H, member 3
GO Process (33)
GO Function (7)
GO Component (5)
Gene Ontology Biological Process
- apoptotic cell clearance [IMP]
- cellular response to lipopolysaccharide [IDA]
- cholesterol homeostasis [ISS]
- gene expression [TAS]
- intracellular receptor signaling pathway [TAS]
- lipid homeostasis [ISS]
- negative regulation of cholesterol storage [IMP]
- negative regulation of inflammatory response [ISS]
- negative regulation of interferon-gamma-mediated signaling pathway [NAS]
- negative regulation of lipid transport [IMP]
- negative regulation of macrophage activation [ISS]
- negative regulation of macrophage derived foam cell differentiation [IC]
- negative regulation of pancreatic juice secretion [ISS]
- negative regulation of pinocytosis [IMP]
- negative regulation of secretion of lysosomal enzymes [ISS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of cellular protein metabolic process [IMP]
- positive regulation of cholesterol efflux [IDA, IMP]
- positive regulation of cholesterol homeostasis [IDA]
- positive regulation of cholesterol transport [IDA]
- positive regulation of fatty acid biosynthetic process [IMP]
- positive regulation of lipoprotein lipase activity [IMP]
- positive regulation of receptor biosynthetic process [IDA]
- positive regulation of toll-like receptor 4 signaling pathway [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IMP]
- positive regulation of triglyceride biosynthetic process [IMP]
- regulation of cholesterol homeostasis [ISS]
- regulation of circadian rhythm [TAS]
- response to progesterone [IDA]
- sterol homeostasis [ISS]
- transcription initiation from RNA polymerase II promoter [TAS]
- triglyceride homeostasis [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-Luminescence
An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.
Publication
Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition.
HSP90 is a molecular chaperone that associates with numerous substrate proteins called clients. It plays many important roles in human biology and medicine, but determinants of client recognition by HSP90 have remained frustratingly elusive. We systematically and quantitatively surveyed most human kinases, transcription factors, and E3 ligases for interaction with HSP90 and its cochaperone CDC37. Unexpectedly, many more kinases than ... [more]
Cell Aug. 31, 2012; 150(5);987-1001 [Pubmed: 22939624]
Throughput
- Low Throughput
Curated By
- BioGRID