BAIT
HSP90AA1
EL52, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP-2, LAP2
heat shock protein 90kDa alpha (cytosolic), class A member 1
GO Process (16)
GO Function (10)
GO Component (10)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- axon guidance [TAS]
- chaperone-mediated protein complex assembly [IDA]
- innate immune response [TAS]
- mitochondrial transport [TAS]
- mitotic cell cycle [TAS]
- nitric oxide metabolic process [TAS]
- positive regulation of nitric oxide biosynthetic process [ISS]
- protein import into mitochondrial outer membrane [IDA]
- protein refolding [TAS]
- regulation of nitric-oxide synthase activity [TAS]
- response to unfolded protein [NAS]
- signal transduction [NAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PRKD2
PKD2, nPKC-D2, HSPC187
protein kinase D2
GO Process (31)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- T cell receptor signaling pathway [IDA]
- cell death [IMP]
- cellular response to vascular endothelial growth factor stimulus [IGI, IMP]
- endothelial tube morphogenesis [TAS]
- intracellular signal transduction [IMP]
- peptidyl-serine phosphorylation [IDA]
- positive regulation of CREB transcription factor activity [IGI]
- positive regulation of DNA biosynthetic process [ISS]
- positive regulation of ERK1 and ERK2 cascade [ISS]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of T cell receptor signaling pathway [ISS]
- positive regulation of angiogenesis [IGI, IMP]
- positive regulation of blood vessel endothelial cell migration [IGI, IMP]
- positive regulation of cell adhesion [IMP]
- positive regulation of endothelial cell chemotaxis [IMP]
- positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway [IGI, IMP]
- positive regulation of endothelial cell migration [IMP]
- positive regulation of endothelial cell proliferation [IGI, IMP]
- positive regulation of fibroblast growth factor receptor signaling pathway [IMP]
- positive regulation of histone deacetylase activity [IGI]
- positive regulation of interleukin-2 production [ISS]
- positive regulation of interleukin-8 production [IMP]
- positive regulation of intracellular signal transduction [IMP]
- positive regulation of peptidyl-serine phosphorylation [IGI]
- positive regulation of sequence-specific DNA binding transcription factor activity [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of vascular endothelial growth factor receptor signaling pathway [IMP]
- protein autophosphorylation [IDA, TAS]
- protein kinase D signaling [IGI]
- protein phosphorylation [NAS]
- vascular endothelial growth factor receptor signaling pathway [IMP]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-Luminescence
An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.
Publication
Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition.
HSP90 is a molecular chaperone that associates with numerous substrate proteins called clients. It plays many important roles in human biology and medicine, but determinants of client recognition by HSP90 have remained frustratingly elusive. We systematically and quantitatively surveyed most human kinases, transcription factors, and E3 ligases for interaction with HSP90 and its cochaperone CDC37. Unexpectedly, many more kinases than ... [more]
Cell Aug. 31, 2012; 150(5);987-1001 [Pubmed: 22939624]
Throughput
- High Throughput
Curated By
- BioGRID