An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

FCHo proteins are nucleators of clathrin-mediated endocytosis.

Henne WM, Boucrot E, Meinecke M, Evergren E, Vallis Y, Mittal R, McMahon HT

Clathrin-mediated endocytosis, the major pathway for ligand internalization into eukaryotic cells, is thought to be initiated by the clustering of clathrin and adaptors around receptors destined for internalization. However, here we report that the membrane-sculpting F-BAR domain-containing Fer/Cip4 homology domain-only proteins 1 and 2 (FCHo1/2) were required for plasma membrane clathrin-coated vesicle (CCV) budding and marked sites of CCV formation. ... [more]

Science Jun. 04, 2010; 328(5983);1281-4 [Pubmed: 20448150]

Throughput

Low Throughput

Additional Notes

figure S6.

Curated By

BioGRID

Interaction Summary

FCHO2

Gene Ontology Biological Process

Gene Ontology Molecular Function

phosphatidylinositol binding [IDA]phosphatidylinositol-4,5-bisphosphate binding [IDA]phosphatidylserine binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

EPS15L1