BAIT

ZFP36L1

BRF1, Berg36, ERF-1, ERF1, RNF162B, TIS11B, cMG1
ZFP36 ring finger protein-like 1
Homo sapiens
PREY

YWHAB

GW128, HEL-S-1, HS1, KCIP-1, YWHAA, RP1-148E22.1
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

BRF1 protein turnover and mRNA decay activity are regulated by protein kinase B at the same phosphorylation sites.

Benjamin D, Schmidlin M, Min L, Gross B, Moroni C

BRF1 posttranscriptionally regulates mRNA levels by targeting ARE-bearing transcripts to the decay machinery. We previously showed that protein kinase B (PKB) phosphorylates BRF1 at Ser92, resulting in binding to 14-3-3 and impairment of mRNA decay activity. Here we identify an additional regulatory site at Ser203 that cooperates in vivo with Ser92. In vitro kinase labeling and wortmannin sensitivity indicate that ... [more]

Mol. Cell. Biol. Dec. 01, 2006; 26(24);9497-507 [Pubmed: 17030608]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
YWHAB ZFP36L1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9903BioGRID
3235984

Curated By

  • BioGRID