BAIT
PPP5C
PP5, PPP5, PPT
protein phosphatase 5, catalytic subunit
GO Process (4)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CCT4
CCT-DELTA, Cctd, SRB
chaperonin containing TCP1, subunit 4 (delta)
GO Process (3)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Label-free quantitative proteomics and SAINT analysis enable interactome mapping for the human Ser/Thr protein phosphatase 5.
Affinity purification coupled to mass spectrometry (AP-MS) represents a powerful and proven approach for the analysis of protein-protein interactions. However, the detection of true interactions for proteins that are commonly considered background contaminants is currently a limitation of AP-MS. Here using spectral counts and the new statistical tool, Significance Analysis of INTeractome (SAINT), true interaction between the serine/threonine protein phosphatase ... [more]
Proteomics Apr. 01, 2011; 11(8);1508-16 [Pubmed: 21360678]
Throughput
- Low Throughput
Curated By
- BioGRID