An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Label-free quantitative proteomics and SAINT analysis enable interactome mapping for the human Ser/Thr protein phosphatase 5.

Skarra DV, Goudreault M, Choi H, Mullin M, Nesvizhskii AI, Gingras AC, Honkanen RE

Affinity purification coupled to mass spectrometry (AP-MS) represents a powerful and proven approach for the analysis of protein-protein interactions. However, the detection of true interactions for proteins that are commonly considered background contaminants is currently a limitation of AP-MS. Here using spectral counts and the new statistical tool, Significance Analysis of INTeractome (SAINT), true interaction between the serine/threonine protein phosphatase ... [more]

Proteomics Apr. 01, 2011; 11(8);1508-16 [Pubmed: 21360678]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

HSP90AA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [TAS]ATPase activity [IDA]MHC class II protein complex binding [IDA]TPR domain binding [IDA, TAS]identical protein binding [IPI]nitric-oxide synthase regulator activity [IDA]nucleotide binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein homodimerization activity [TAS]

Gene Ontology Cellular Component

SSBP1