TNK2
Gene Ontology Biological Process
- cell differentiation [IBA]
- cell migration [IBA]
- cell surface receptor signaling pathway [TAS]
- innate immune response [IBA]
- negative regulation of catalytic activity [TAS]
- peptidyl-tyrosine autophosphorylation [IBA]
- phosphorylation [IDA]
- positive regulation of peptidyl-tyrosine phosphorylation [IDA]
- regulation of cell proliferation [IBA]
- regulation of clathrin-mediated endocytosis [IDA]
- small GTPase mediated signal transduction [TAS]
- transmembrane receptor protein tyrosine kinase signaling pathway [IBA]
Gene Ontology Molecular Function- GTPase inhibitor activity [TAS]
- WW domain binding [ISS]
- epidermal growth factor receptor binding [IDA]
- hormone receptor binding [IBA]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- protein binding [IPI]
- protein serine/threonine/tyrosine kinase activity [IDA]
- protein tyrosine kinase activity [IDA]
- GTPase inhibitor activity [TAS]
- WW domain binding [ISS]
- epidermal growth factor receptor binding [IDA]
- hormone receptor binding [IBA]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- protein binding [IPI]
- protein serine/threonine/tyrosine kinase activity [IDA]
- protein tyrosine kinase activity [IDA]
Gene Ontology Cellular Component
TNK2
Gene Ontology Biological Process
- cell differentiation [IBA]
- cell migration [IBA]
- cell surface receptor signaling pathway [TAS]
- innate immune response [IBA]
- negative regulation of catalytic activity [TAS]
- peptidyl-tyrosine autophosphorylation [IBA]
- phosphorylation [IDA]
- positive regulation of peptidyl-tyrosine phosphorylation [IDA]
- regulation of cell proliferation [IBA]
- regulation of clathrin-mediated endocytosis [IDA]
- small GTPase mediated signal transduction [TAS]
- transmembrane receptor protein tyrosine kinase signaling pathway [IBA]
Gene Ontology Molecular Function- GTPase inhibitor activity [TAS]
- WW domain binding [ISS]
- epidermal growth factor receptor binding [IDA]
- hormone receptor binding [IBA]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- protein binding [IPI]
- protein serine/threonine/tyrosine kinase activity [IDA]
- protein tyrosine kinase activity [IDA]
- GTPase inhibitor activity [TAS]
- WW domain binding [ISS]
- epidermal growth factor receptor binding [IDA]
- hormone receptor binding [IBA]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- protein binding [IPI]
- protein serine/threonine/tyrosine kinase activity [IDA]
- protein tyrosine kinase activity [IDA]
Gene Ontology Cellular Component
Biochemical Activity (Phosphorylation)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Purification and enzyme activity of ACK1.
The activated Cdc42 associated kinases (ACKs) are nonreceptor tyrosine kinases that are specific targets of Cdc42. To study the biochemical properties of ACK1, we expressed and purified the enzyme using the baculovirus/Sf9 cell system. This ACK1 construct contains (from N- to C-terminus) the kinase catalytic domain, SH3 domain, and Cdc42-binding CRIB domain. We describe enzyme activity assays based on synthetic ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 2.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TNK2 TNK2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| TNK2 TNK2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TNK2 TNK2 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 3457756 | |
| TNK2 TNK2 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 724116 | |
| TNK2 TNK2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID