MTA1
Gene Ontology Biological Process
- circadian regulation of gene expression [IMP]
- double-strand break repair [IMP, ISO]
- entrainment of circadian clock by photoperiod [IMP]
- locomotor rhythm [IMP]
- negative regulation of nucleic acid-templated transcription [IMP, ISO]
- positive regulation of protein autoubiquitination [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- regulation of gene expression, epigenetic [ISO]
- regulation of inflammatory response [IMP]
- regulation of nucleic acid-templated transcription [IDA]
- response to ionizing radiation [IDA, ISO]
- response to lipopolysaccharide [IDA]
- secretory granule organization [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SH3GL3
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Direct interaction between metastasis-associated protein 1 and endophilin 3.
The yeast two-hybrid system was used to search for partners of mouse metastasis-associated protein 1 (Mta1). Screening of a cDNA library prepared from mouse embryo yielded positive clones coding for endophilin 3. The site of interaction was suggested to be the SH-3-binding domain of Mta1 and SH-3 domain of endophilin 3. This interaction was confirmed by GST pull-down assay in ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
MTA1 SH3GL3 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID