Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic analysis of α4β1 integrin adhesion complexes reveals α-subunit-dependent protein recruitment.

Byron A, Humphries JD, Craig SE, Knight D, Humphries MJ

Integrin adhesion receptors mediate cell-cell and cell-extracellular matrix interactions, which control cell morphology and migration, differentiation, and tissue integrity. Integrins recruit multimolecular adhesion complexes to their cytoplasmic domains, which provide structural and mechanosensitive signaling connections between the extracellular and intracellular milieux. The different functions of specific integrin heterodimers, such as α4β1 and α5β1, have been attributed to distinct signal transduction ... [more]

Proteomics Jul. 01, 2012; 12(13);2107-14 [Pubmed: 22623428]

Throughput

  • Low Throughput

Ontology Terms

  • disease: chronic myeloid leukemia (DOID:8552)
  • cell line: k-562 cell (BTO:0000664)

Additional Notes

  • exogenous expression of bait

Curated By

  • BioGRID