ARRB2
Gene Ontology Biological Process
- G-protein coupled receptor internalization [IDA, IMP]
- Notch signaling pathway [TAS]
- blood coagulation [TAS]
- cell chemotaxis [IMP]
- desensitization of G-protein coupled receptor protein signaling pathway by arrestin [IMP]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of natural killer cell mediated cytotoxicity [IMP]
- negative regulation of protein ubiquitination [IDA]
- platelet activation [TAS]
- positive regulation of ERK1 and ERK2 cascade [IDA, IMP]
- positive regulation of protein ubiquitination [IGI]
- positive regulation of receptor internalization [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein ubiquitination [IMP]
- receptor internalization [IDA]
- regulation of androgen receptor signaling pathway [IDA]
- transcription from RNA polymerase II promoter [IDA]
- transforming growth factor beta receptor signaling pathway [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EDNRA
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [NAS]
- activation of adenylate cyclase activity [TAS]
- activation of phospholipase C activity [TAS]
- artery smooth muscle contraction [IMP]
- cell proliferation [NAS]
- endothelin receptor signaling pathway [NAS]
- glucose transport [ISS]
- metabolic process [TAS]
- positive regulation of cytosolic calcium ion concentration [TAS]
- respiratory gaseous exchange [ISS]
- signal transduction [TAS]
- smooth muscle contraction [NAS]
- vasoconstriction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Beta-arrestin links endothelin A receptor to beta-catenin signaling to induce ovarian cancer cell invasion and metastasis.
The activation of endothelin-A receptor (ET(A)R) by endothelin-1 (ET-1) has a critical role in ovarian tumorigenesis and progression. To define the molecular mechanism in ET-1-induced tumor invasion and metastasis, we focused on beta-arrestins as scaffold and signaling proteins of G protein-coupled receptors. Here, we demonstrate that, in ovarian cancer cells, beta-arrestin is recruited to ET(A)R to form two trimeric complexes: ... [more]
Throughput
- Low Throughput
Ontology Terms
- cell line: hey cell (BTO:0002590)
- cell line: ovca-433 cell (BTO:0002566)
Additional Notes
- Complex between beta-arrestins, EDNRA, and Src forms when cells treated with endothelin (ligand for EDNRA)
- figure 1.
Curated By
- BioGRID