BAIT
HSPB1
27kDa, Hsp25
heat shock protein 1
GO Process (15)
GO Function (6)
GO Component (10)
Gene Ontology Biological Process
- cellular response to vascular endothelial growth factor stimulus [ISO]
- intracellular signal transduction [ISO]
- negative regulation of apoptotic signaling pathway [IDA]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [IDA]
- negative regulation of protein kinase activity [IDA]
- negative regulation of protein serine/threonine kinase activity [IDA]
- platelet aggregation [ISO]
- positive regulation of angiogenesis [ISO]
- positive regulation of blood vessel endothelial cell migration [ISO]
- positive regulation of endothelial cell chemotaxis [ISO]
- positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway [ISO]
- positive regulation of interleukin-1 beta production [IDA]
- positive regulation of tumor necrosis factor biosynthetic process [IDA]
- regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- response to unfolded protein [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
INS
IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
insulin
GO Process (60)
GO Function (6)
GO Component (6)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- MAPK cascade [IDA]
- activation of protein kinase B activity [IDA]
- acute-phase response [IDA]
- alpha-beta T cell activation [IDA]
- cell-cell signaling [IC]
- cellular protein metabolic process [TAS]
- endocrine pancreas development [TAS]
- energy reserve metabolic process [TAS]
- fatty acid homeostasis [IMP]
- glucose homeostasis [IMP]
- glucose transport [IDA]
- insulin receptor signaling pathway [TAS]
- negative regulation of NAD(P)H oxidase activity [IDA]
- negative regulation of acute inflammatory response [IDA]
- negative regulation of fatty acid metabolic process [IMP]
- negative regulation of feeding behavior [IDA]
- negative regulation of gluconeogenesis [NAS]
- negative regulation of glycogen catabolic process [IMP]
- negative regulation of lipid catabolic process [IMP, NAS]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [NAS]
- negative regulation of protein catabolic process [IDA]
- negative regulation of protein oligomerization [IDA]
- negative regulation of protein secretion [IDA]
- negative regulation of proteolysis [IMP]
- negative regulation of respiratory burst involved in inflammatory response [IDA]
- negative regulation of vasodilation [NAS]
- positive regulation of DNA replication [IDA]
- positive regulation of MAPK cascade [IDA]
- positive regulation of NF-kappaB transcription factor activity [IDA]
- positive regulation of brown fat cell differentiation [TAS]
- positive regulation of cell differentiation [NAS]
- positive regulation of cell growth [NAS]
- positive regulation of cell migration [ISS]
- positive regulation of cell proliferation [IDA]
- positive regulation of cellular protein metabolic process [IMP]
- positive regulation of cytokine secretion [IDA]
- positive regulation of glucose import [IDA]
- positive regulation of glycogen biosynthetic process [IDA]
- positive regulation of glycolytic process [IDA, IMP]
- positive regulation of insulin receptor signaling pathway [IDA]
- positive regulation of lipid biosynthetic process [NAS]
- positive regulation of mitosis [IDA]
- positive regulation of nitric oxide biosynthetic process [NAS]
- positive regulation of nitric-oxide synthase activity [NAS]
- positive regulation of peptide hormone secretion [TAS]
- positive regulation of peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of phosphatidylinositol 3-kinase signaling [IDA]
- positive regulation of protein autophosphorylation [ISS]
- positive regulation of protein kinase B signaling [IDA]
- positive regulation of respiratory burst [IDA]
- positive regulation of vasodilation [NAS]
- regulation of cellular amino acid metabolic process [IMP]
- regulation of insulin secretion [TAS]
- regulation of protein localization [IDA]
- regulation of protein secretion [IDA]
- regulation of transcription, DNA-templated [NAS]
- regulation of transmembrane transporter activity [IDA]
- small molecule metabolic process [TAS]
- wound healing [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Regulation of Hsp27 oligomerization, chaperone function, and protective activity against oxidative stress/tumor necrosis factor alpha by phosphorylation.
The small heat shock proteins (sHsps) from human (Hsp27) and mouse (Hsp25) form large oligomers which can act as molecular chaperones in vitro and protect cells from heat shock and oxidative stress when overexpressed. In addition, mammalian sHsps are rapidly phosphorylated by MAPKAP kinase 2/3 at two or three serine residues in response to various extracellular stresses. Here we analyze ... [more]
J. Biol. Chem. Jul. 02, 1999; 274(27);18947-56 [Pubmed: 10383393]
Throughput
- Low Throughput
Curated By
- BioGRID