Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability.

Bett JS, Ibrahim AF, Garg AK, Kelly V, Pedrioli P, Rocha S, Hay RT

HIF1A (hypoxia-inducible factor 1α) is the master regulator of the cellular response to hypoxia and is implicated in cancer progression. Whereas the regulation of HIF1A protein in response to oxygen is well characterized, less is known about the fate of HIF1A mRNA. In the present study, we have identified the pseudo-DUB (deubiquitinating enzyme)/deadenylase USP52 (ubiquitin-specific protease 52)/PAN2 [poly(A) nuclease 2] ... [more]

Biochem. J. Apr. 15, 2013; 451(2);185-94 [Pubmed: 23398456]

Throughput

  • High Throughput

Ontology Terms

  • cell line: hek-293 cell (BTO:0000007)

Additional Notes

  • exogenous expression of bait

Curated By

  • BioGRID