An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability.

Bett JS, Ibrahim AF, Garg AK, Kelly V, Pedrioli P, Rocha S, Hay RT

HIF1A (hypoxia-inducible factor 1Î±) is the master regulator of the cellular response to hypoxia and is implicated in cancer progression. Whereas the regulation of HIF1A protein in response to oxygen is well characterized, less is known about the fate of HIF1A mRNA. In the present study, we have identified the pseudo-DUB (deubiquitinating enzyme)/deadenylase USP52 (ubiquitin-specific protease 52)/PAN2 [poly(A) nuclease 2] ... [more]

Biochem. J. Apr. 15, 2013; 451(2);185-94 [Pubmed: 23398456]

Throughput

High Throughput

Ontology Terms

cell line: hek-293 cell (BTO:0000007)

Additional Notes

exogenous expression of bait

Curated By

BioGRID

Interaction Summary

PAN2

Gene Ontology Biological Process

Gene Ontology Molecular Function

3'-5'-exoribonuclease activity [IDA]poly(A)-specific ribonuclease activity [IDA, TAS]protein binding [IPI]

Gene Ontology Cellular Component

HNRNPM

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [NAS]poly(A) RNA binding [IDA]protein binding [IPI]protein domain specific binding [IPI]