PREY

PPP1R9A

Neb1
protein phosphatase 1, regulatory subunit 9A
GO Process (3)
GO Function (2)
GO Component (6)
Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Rac3-induced neuritogenesis requires binding to Neurabin I.

Orioli D, Colaluca IN, Stefanini M, Riva S, Dotti CG, Peverali FA

Rac3, a neuronal GTP-binding protein of the Rho family, induces neuritogenesis in primary neurons. Using yeast two-hybrid analysis, we show that Neurabin I, the neuronal F-actin binding protein, is a direct Rac3-interacting molecule. Biochemical and light microscopy studies indicate that Neurabin I copartitions and colocalizes with Rac3 at the growth cones of neurites, inducing Neurabin I association to the cytoskeleton. ... [more]

Mol. Biol. Cell May. 01, 2006; 17(5);2391-400 [Pubmed: 16525025]

Throughput

  • Low Throughput

Curated By

  • BioGRID