BAIT

MUSK

Mdk4, Mlk, Nsk1, Nsk2, Nsk3, RP23-170A3.1
muscle, skeletal, receptor tyrosine kinase
Mus musculus
PREY

DOK7

C4orf25, CMS1B, RP11-529E10.4
docking protein 7
GO Process (1)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The muscle protein Dok-7 is essential for neuromuscular synaptogenesis.

Okada K, Inoue A, Okada M, Murata Y, Kakuta S, Jigami T, Kubo S, Shiraishi H, Eguchi K, Motomura M, Akiyama T, Iwakura Y, Higuchi O, Yamanashi Y

The formation of the neuromuscular synapse requires muscle-specific receptor kinase (MuSK) to orchestrate postsynaptic differentiation, including the clustering of receptors for the neurotransmitter acetylcholine. Upon innervation, neural agrin activates MuSK to establish the postsynaptic apparatus, although agrin-independent formation of neuromuscular synapses can also occur experimentally in the absence of neurotransmission. Dok-7, a MuSK-interacting cytoplasmic protein, is essential for MuSK activation ... [more]

Science Jun. 23, 2006; 312(5781);1802-5 [Pubmed: 16794080]

Throughput

  • Low Throughput

Curated By

  • BioGRID