BAIT

LCK

IMD22, LSK, YT16, p56lck, pp58lck, RP4-675E8.4
LCK proto-oncogene, Src family tyrosine kinase
GO Process (30)
GO Function (14)
GO Component (7)
Homo sapiens

Biochemical Activity (Phosphorylation)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Protein-tyrosine kinase and GTPase signals cooperate to phosphorylate and activate Wiskott-Aldrich syndrome protein (WASP)/neuronal WASP.

Torres E, Rosen MK

Protein-tyrosine kinases and Rho GTPases regulate many cellular processes, including the reorganization and dynamics of the actin cytoskeleton. The Wiskott-Aldrich syndrome protein (WASP) and its homolog neuronal WASP (N-WASP) are effectors of the Rho GTPase Cdc42 and provide a direct link between activated membrane receptors and the actin cytoskeleton. WASP and N-WASP are also regulated by a large number of ... [more]

J. Biol. Chem. Feb. 10, 2006; 281(6);3513-20 [Pubmed: 16293614]

Throughput

  • Low Throughput

Additional Notes

  • GBD 12x104
  • GBD-P-VCA 0.32x104
  • GBD-P-VCA+Cdc42 8.6x104
  • GBD-VCA 0.28x104
  • GBD-VCA+Cdc42 3.7x104
  • Kcat/Km (M-1 s-1)
  • Lck phosphorylation of WASP/N-WASP domains

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LCK WAS
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
727214

Curated By

  • BioGRID