STK4
Gene Ontology Biological Process
- apoptotic process [IDA]
- cell morphogenesis [IDA]
- hippo signaling [IDA, TAS]
- intracellular signal transduction [IDA]
- negative regulation of canonical Wnt signaling pathway [IMP]
- peptidyl-serine phosphorylation [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of fat cell differentiation [IGI]
- positive regulation of protein binding [IDA]
- positive regulation of protein serine/threonine kinase activity [TAS]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA]
- signal transduction [TAS]
- signal transduction by phosphorylation [IBA]
Gene Ontology Molecular Function- ATP binding [IDA]
- identical protein binding [IPI]
- magnesium ion binding [IDA]
- protein binding [IPI]
- protein dimerization activity [IDA]
- protein homodimerization activity [IDA]
- protein serine/threonine kinase activator activity [TAS]
- protein serine/threonine kinase activity [EXP, IDA]
- receptor signaling protein serine/threonine kinase activity [IBA]
- transcription factor binding [IPI]
- ATP binding [IDA]
- identical protein binding [IPI]
- magnesium ion binding [IDA]
- protein binding [IPI]
- protein dimerization activity [IDA]
- protein homodimerization activity [IDA]
- protein serine/threonine kinase activator activity [TAS]
- protein serine/threonine kinase activity [EXP, IDA]
- receptor signaling protein serine/threonine kinase activity [IBA]
- transcription factor binding [IPI]
AKT1
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- G-protein coupled receptor signaling pathway [TAS]
- RNA metabolic process [TAS]
- T cell costimulation [TAS]
- activation-induced cell death of T cells [IMP]
- apoptotic process [TAS]
- blood coagulation [TAS]
- cell differentiation [TAS]
- cell proliferation [TAS]
- cellular protein modification process [TAS]
- cellular response to insulin stimulus [IMP, ISS]
- endocrine pancreas development [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [IMP]
- insulin-like growth factor receptor signaling pathway [IMP]
- intracellular signal transduction [IDA]
- intrinsic apoptotic signaling pathway [TAS]
- mRNA metabolic process [TAS]
- mammary gland epithelial cell differentiation [TAS]
- membrane organization [TAS]
- negative regulation of apoptotic process [IDA]
- negative regulation of autophagy [IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISS]
- negative regulation of endopeptidase activity [IMP]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [TAS]
- negative regulation of fatty acid beta-oxidation [IMP]
- negative regulation of neuron death [NAS]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [NAS]
- negative regulation of plasma membrane long-chain fatty acid transport [IMP]
- negative regulation of protein kinase activity [IMP, ISS]
- negative regulation of proteolysis [IMP]
- negative regulation of release of cytochrome c from mitochondria [ISS]
- neurotrophin TRK receptor signaling pathway [TAS]
- nitric oxide biosynthetic process [TAS]
- nitric oxide metabolic process [TAS]
- peptidyl-serine phosphorylation [IDA]
- phosphatidylinositol-mediated signaling [TAS]
- phosphorylation [IDA]
- platelet activation [TAS]
- positive regulation of blood vessel endothelial cell migration [IDA]
- positive regulation of cell growth [IDA]
- positive regulation of cellular protein metabolic process [ISS]
- positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle [IDA]
- positive regulation of endothelial cell proliferation [IMP]
- positive regulation of establishment of protein localization to plasma membrane [IMP]
- positive regulation of fat cell differentiation [IMP]
- positive regulation of glucose import [IMP]
- positive regulation of glucose metabolic process [IMP]
- positive regulation of glycogen biosynthetic process [IMP, NAS]
- positive regulation of lipid biosynthetic process [IMP]
- positive regulation of nitric oxide biosynthetic process [IMP]
- positive regulation of nitric-oxide synthase activity [IMP]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- protein autophosphorylation [TAS]
- protein import into nucleus, translocation [IMP]
- protein phosphorylation [IDA]
- regulation of cell cycle checkpoint [TAS]
- regulation of cell migration [IMP, TAS]
- regulation of glycogen biosynthetic process [IMP]
- regulation of neuron projection development [ISS]
- regulation of nitric-oxide synthase activity [TAS]
- response to UV-A [IDA]
- response to fluid shear stress [IMP]
- response to heat [TAS]
- response to oxidative stress [ISS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- 14-3-3 protein binding [IPI]
- ATP binding [IC, IDA]
- enzyme binding [ISS]
- identical protein binding [IPI]
- kinase activity [IDA]
- nitric-oxide synthase regulator activity [IMP]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- phosphatidylinositol-3,4-bisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [IDA, TAS]
- protein serine/threonine/tyrosine kinase activity [IDA]
- 14-3-3 protein binding [IPI]
- ATP binding [IC, IDA]
- enzyme binding [ISS]
- identical protein binding [IPI]
- kinase activity [IDA]
- nitric-oxide synthase regulator activity [IMP]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- phosphatidylinositol-3,4-bisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [IDA, TAS]
- protein serine/threonine/tyrosine kinase activity [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The pro-apoptotic kinase Mst1 and its caspase cleavage products are direct inhibitors of Akt1.
Akt kinases mediate cell growth and survival. Here, we report that a pro-apoptotic kinase, Mst1/STK4, is a physiological Akt1 interaction partner. Mst1 was identified as a component of an Akt1 multiprotein complex isolated from lipid raft-enriched fractions of LNCaP human prostate cancer cells. Endogenous Mst1, along with its paralog, Mst2, acted as inhibitors of endogenous Akt1. Surprisingly, mature Mst1 as ... [more]
Throughput
- Low Throughput
Additional Notes
- STK4 interacts with wildtype and myristoylated Akt1
- both deletion mutants containing Mst1-N-terminal or Mst1-C-terminal domains interacted with myristoylated and wildtype Akt1
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AKT1 STK4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 869915 | |
| AKT1 STK4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 869916 | |
| AKT1 STK4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| STK4 AKT1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID