BAIT

PUF3

mRNA-binding protein PUF3, YLL013C
Protein of the mitochondrial outer surface; links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

ORC6

origin recognition complex subunit 6, L000001306, YHR118C
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p; mutation in the human Orc6p is linked to Meier-Gorlin syndrome
Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Pervasive and dynamic protein binding sites of the mRNA transcriptome in Saccharomyces cerevisiae.

Freeberg MA, Han T, Moresco JJ, Kong A, Yang YC, Lu ZJ, Yates JR, Kim JK

ABSTRACT: BACKGROUND: Protein-RNA interactions are integral components of nearly every aspect of biology including regulation of gene expression, assembly of cellular architectures, and pathogenesis of human diseases. However, studies in the past few decades have only uncovered a small fraction of the vast landscape of the protein-RNA interactome in any organism, and even less is known about the dynamics of ... [more]

Genome Biol. Feb. 14, 2013; 14(2);R13 [Pubmed: 23409723]

Throughput

  • High Throughput

Additional Notes

  • Puf3 protein bound to RNA subdomain: CDS

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ORC6 PUF3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2048BioGRID
1988485

Curated By

  • BioGRID