A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria.

Dujeancourt L, Richter R, Chrzanowska-Lightowlers ZM, Bonnefoy N, Herbert CJ

Mitochondrial translation synthesizes key subunits of the respiratory complexes. In Schizosaccharomyces pombe, strains lacking Mrf1, the mitochondrial stop codon recognition factor, are viable, suggesting that other factors can play a role in translation termination. S. pombe contains four predicted peptidyl tRNA hydrolases, two of which (Pth3 and Pth4), have a GGQ motif that is conserved in class I release factors. ... [more]

Mitochondrion Jul. 24, 2013; 0(0); [Pubmed: 23892058]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Additional Notes

when the pth4D and mrf1D strains were crossed, no viable double mutants were obtained

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MRF1 PTH4	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Dujeancourt L (2013)	Low	-	BioGRID	884922

Curated By

BioGRID

Interaction Summary

MRF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

translation release factor activity [ISS]

Gene Ontology Cellular Component

PTH4