BAIT
P2RX4
purinergic receptor P2X, ligand-gated ion channel 4
GO Process (24)
GO Function (9)
GO Component (16)
Gene Ontology Biological Process
- apoptotic signaling pathway [ISO]
- calcium ion transport [ISO]
- cation transmembrane transport [IDA, ISO]
- cellular response to ATP [ISO, ISS]
- ion transmembrane transport [ISO]
- membrane depolarization [ISO]
- negative regulation of cardiac muscle hypertrophy [ISO]
- neuronal action potential [IDA]
- nitric oxide biosynthetic process [ISO]
- positive regulation of calcium ion transport into cytosol [ISO]
- positive regulation of calcium-mediated signaling [ISO]
- protein homooligomerization [IDA]
- purinergic nucleotide receptor signaling pathway [ISO]
- regulation of blood pressure [ISO]
- regulation of cardiac muscle contraction [ISO]
- regulation of excitatory postsynaptic membrane potential [ISO]
- regulation of sodium ion transport [IDA]
- relaxation of cardiac muscle [ISO]
- response to ATP [ISO]
- response to fluid shear stress [ISO]
- sensory perception of pain [IMP]
- signal transduction [ISO]
- transport [ISO]
- vasodilation [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- apical part of cell [ISO]
- axon [IDA]
- cell junction [ISO]
- dendrite [IDA]
- dendritic spine [IDA]
- extracellular vesicular exosome [ISO]
- integral component of nuclear inner membrane [IBA]
- integral component of plasma membrane [ISO, ISS]
- lysosomal membrane [ISO]
- membrane [ISO]
- neuronal cell body [IDA]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA]
- postsynaptic density [IDA]
- synapse [IDA]
- terminal bouton [IDA]
Rattus norvegicus
PREY
P2RX7
purinergic receptor P2X, ligand-gated ion channel, 7
GO Process (78)
GO Function (13)
GO Component (17)
Gene Ontology Biological Process
- NAD transport [ISO]
- T cell homeostasis [ISO]
- T cell proliferation [ISO]
- activation of MAPK activity [ISO]
- apoptotic signaling pathway [IDA]
- bleb assembly [IDA, ISO]
- calcium ion transport [ISO]
- cation transmembrane transport [IDA, ISO, TAS]
- cation transport [ISO]
- cell morphogenesis [ISO]
- cell surface receptor signaling pathway [IDA]
- cell volume homeostasis [ISO]
- cellular response to extracellular stimulus [ISO]
- ceramide biosynthetic process [ISO]
- collagen metabolic process [ISO]
- cytolysis [ISO]
- defense response to Gram-positive bacterium [ISO]
- extrinsic apoptotic signaling pathway [ISO]
- gene expression [ISO]
- homeostasis of number of cells within a tissue [ISO]
- inflammatory response [ISO]
- membrane budding [ISO]
- membrane depolarization [IDA, ISO]
- membrane protein ectodomain proteolysis [ISO]
- mitochondrion organization [ISO]
- multicellular organismal protein catabolic process [ISO]
- negative regulation of MAPK cascade [ISO]
- negative regulation of bone resorption [ISO]
- neuronal action potential [IDA]
- organic cation transport [TAS]
- phagolysosome assembly [ISO]
- phospholipid transfer to membrane [ISO]
- phospholipid translocation [ISO]
- plasma membrane organization [ISO]
- pore complex assembly [IDA, IMP, ISO]
- positive regulation of MAPK cascade [ISO]
- positive regulation of T cell mediated cytotoxicity [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of bone mineralization [ISO]
- positive regulation of calcium ion transport into cytosol [ISO]
- positive regulation of catalytic activity [ISO]
- positive regulation of cytokine secretion [ISO]
- positive regulation of cytolysis [IDA]
- positive regulation of cytoskeleton organization [IDA]
- positive regulation of gamma-aminobutyric acid secretion [ISO]
- positive regulation of glutamate secretion [ISO]
- positive regulation of interleukin-1 alpha secretion [ISO]
- positive regulation of interleukin-1 beta production [ISO]
- positive regulation of interleukin-1 beta secretion [IDA, IMP, ISO]
- positive regulation of interleukin-6 production [ISO]
- positive regulation of lymphocyte apoptotic process [ISO]
- positive regulation of mitochondrial depolarization [ISO]
- positive regulation of ossification [ISO]
- positive regulation of prostaglandin secretion [ISO]
- positive regulation of protein phosphorylation [IDA, ISO]
- positive regulation of protein secretion [ISO]
- programmed cell death [ISO]
- protein oligomerization [ISO]
- protein phosphorylation [ISO]
- protein processing [ISO]
- purinergic nucleotide receptor signaling pathway [IDA, ISO]
- reactive oxygen species metabolic process [ISO]
- regulation of sodium ion transport [IDA]
- release of sequestered calcium ion into cytosol [ISO]
- response to ATP [IDA, ISO]
- response to bacterium [ISO]
- response to calcium ion [ISO]
- response to drug [ISO]
- response to electrical stimulus [ISO]
- response to fluid shear stress [ISO]
- response to lipopolysaccharide [ISO]
- response to mechanical stimulus [ISO]
- response to organic cyclic compound [ISO]
- response to organic substance [ISO]
- response to zinc ion [ISO]
- sensory perception of pain [ISO]
- skeletal system morphogenesis [ISO]
- synaptic vesicle exocytosis [ISO]
Gene Ontology Molecular Function- ATP binding [IDA, ISO]
- channel activity [IDA, ISO]
- copper ion binding [IDA]
- extracellular ATP-gated cation channel activity [IDA, ISO, TAS]
- lipopolysaccharide binding [ISO]
- magnesium ion binding [IDA]
- protein binding [IPI]
- protein serine/threonine kinase activator activity [IC, IMP]
- purinergic nucleotide receptor activity [IDA, ISO]
- receptor activity [ISO]
- receptor binding [IPI]
- scaffold protein binding [IPI]
- zinc ion binding [IDA]
- ATP binding [IDA, ISO]
- channel activity [IDA, ISO]
- copper ion binding [IDA]
- extracellular ATP-gated cation channel activity [IDA, ISO, TAS]
- lipopolysaccharide binding [ISO]
- magnesium ion binding [IDA]
- protein binding [IPI]
- protein serine/threonine kinase activator activity [IC, IMP]
- purinergic nucleotide receptor activity [IDA, ISO]
- receptor activity [ISO]
- receptor binding [IPI]
- scaffold protein binding [IPI]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
- bleb [IDA]
- cell-cell junction [ISO]
- cytoplasm [IDA]
- cytosol [ISO]
- external side of plasma membrane [ISO]
- integral component of nuclear inner membrane [IDA]
- integral component of plasma membrane [ISO]
- intracellular [ISO]
- membrane [ISO]
- membrane raft [IDA]
- neuromuscular junction [ISO]
- neuron projection [IDA]
- neuronal cell body [ISO]
- plasma membrane [IDA, ISO, TAS]
- protein complex [IDA]
- synapse [ISO]
- terminal bouton [IDA]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Evidence for functional P2X4/P2X7 heteromeric receptors.
The cytolytic ionotropic ATP receptor P2X7 has several important roles in immune cell regulation, such as cytokine release, apoptosis, and microbial killing. Although P2X7 receptors are frequently coexpressed with another subtype of P2X receptor, P2X4, they are believed not to form heteromeric assemblies but to function only as homomers. Both receptors play a role in neuropathic pain; therefore, understanding how ... [more]
Mol. Pharmacol. Dec. 01, 2007; 72(6);1447-56 [Pubmed: 17785580]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| P2RX4 P2RX7 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - |
Curated By
- BioGRID