BAIT
TRAF6
2310003F17Rik, AI851288, C630032O20Rik, RP23-313G3.1
TNF receptor-associated factor 6
GO Process (44)
GO Function (13)
GO Component (12)
Gene Ontology Biological Process
- I-kappaB kinase/NF-kappaB signaling [IMP]
- JNK cascade [ISO]
- T cell receptor signaling pathway [ISO]
- T-helper 1 type immune response [IMP]
- activation of NF-kappaB-inducing kinase activity [ISO]
- activation of protein kinase activity [ISO]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [IMP]
- bone remodeling [IMP]
- bone resorption [IMP]
- cell development [IMP]
- cellular response to lipopolysaccharide [ISO]
- cytokine-mediated signaling pathway [ISO]
- immune response [IMP]
- interleukin-1-mediated signaling pathway [IMP, ISO]
- myeloid dendritic cell differentiation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- neural tube closure [IMP]
- odontogenesis of dentin-containing tooth [IMP]
- organ morphogenesis [IMP]
- ossification [IMP]
- osteoclast differentiation [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IGI, IMP, ISO]
- positive regulation of JUN kinase activity [ISO]
- positive regulation of NF-kappaB transcription factor activity [IMP, ISO]
- positive regulation of T cell cytokine production [ISO]
- positive regulation of T cell proliferation [IMP]
- positive regulation of interleukin-12 biosynthetic process [IMP]
- positive regulation of interleukin-2 production [ISO]
- positive regulation of interleukin-6 biosynthetic process [IMP]
- positive regulation of lipopolysaccharide-mediated signaling pathway [IMP]
- positive regulation of osteoclast differentiation [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- positive regulation of smooth muscle cell proliferation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription regulatory region DNA binding [ISO]
- protein K63-linked ubiquitination [IDA, ISO]
- protein autoubiquitination [ISO, TAS]
- protein complex assembly [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IDA, IGI, IMP]
- regulation of immunoglobulin secretion [IDA]
- response to interleukin-1 [ISO]
- signal transduction [IDA, TAS]
Gene Ontology Molecular Function- histone deacetylase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase B binding [ISO]
- protein kinase binding [ISO]
- signal transducer activity [TAS]
- thioesterase binding [ISO]
- tumor necrosis factor receptor binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, IMP]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
- histone deacetylase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase B binding [ISO]
- protein kinase binding [ISO]
- signal transducer activity [TAS]
- thioesterase binding [ISO]
- tumor necrosis factor receptor binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, IMP]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
TLR4
Lps, Ly87, Ran/M1, Rasl2-8, RP24-209H15.1
toll-like receptor 4
GO Process (71)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
- B cell proliferation involved in immune response [IMP]
- I-kappaB phosphorylation [IMP, ISO]
- activation of MAPK activity [IMP]
- activation of NF-kappaB-inducing kinase activity [NAS]
- activation of innate immune response [IMP]
- cellular response to lipopolysaccharide [IDA, IMP]
- cellular response to lipoteichoic acid [IMP]
- defense response to Gram-negative bacterium [IMP]
- detection of lipopolysaccharide [ISO, TAS]
- innate immune response [TAS]
- innate immune response-activating signal transduction [ISO]
- interferon-gamma production [IGI]
- intestinal epithelial structure maintenance [IMP]
- leukotriene metabolic process [ISO]
- lipopolysaccharide-mediated signaling pathway [IDA, IGI, ISO]
- macrophage activation [ISO, ISS]
- mast cell activation [NAS]
- microglial cell activation [ISO]
- negative regulation of ERK1 and ERK2 cascade [IMP]
- negative regulation of interferon-gamma production [IMP]
- negative regulation of interleukin-17 production [IMP]
- negative regulation of interleukin-23 production [IMP]
- negative regulation of interleukin-6 production [IMP]
- negative regulation of tumor necrosis factor production [IMP]
- nitric oxide production involved in inflammatory response [IMP]
- positive regulation of B cell proliferation [IMP]
- positive regulation of DNA binding [ISO]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- positive regulation of JNK cascade [IDA, IGI]
- positive regulation of MHC class II biosynthetic process [IMP]
- positive regulation of NF-kappaB import into nucleus [ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of chemokine production [IDA, ISO]
- positive regulation of inflammatory response [ISO]
- positive regulation of interferon-alpha production [IDA]
- positive regulation of interferon-beta biosynthetic process [IMP]
- positive regulation of interferon-beta production [IDA]
- positive regulation of interferon-gamma production [IMP]
- positive regulation of interleukin-1 biosynthetic process [NAS]
- positive regulation of interleukin-1 production [IDA]
- positive regulation of interleukin-10 production [IDA]
- positive regulation of interleukin-12 biosynthetic process [ISO]
- positive regulation of interleukin-12 production [IDA]
- positive regulation of interleukin-13 biosynthetic process [NAS]
- positive regulation of interleukin-6 biosynthetic process [NAS]
- positive regulation of interleukin-6 production [IDA, IMP, ISO, NAS]
- positive regulation of interleukin-8 biosynthetic process [ISO]
- positive regulation of interleukin-8 production [ISO]
- positive regulation of lymphocyte proliferation [IMP]
- positive regulation of macrophage cytokine production [IMP]
- positive regulation of nitric oxide biosynthetic process [IMP]
- positive regulation of nitric-oxide synthase biosynthetic process [IDA]
- positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway [IDA]
- positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway [IDA]
- positive regulation of peptidyl-tyrosine phosphorylation [ISO]
- positive regulation of platelet activation [IMP]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- positive regulation of stress-activated MAPK cascade [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of tumor necrosis factor biosynthetic process [ISO]
- positive regulation of tumor necrosis factor production [IDA, IMP]
- regulation of inflammatory response [IMP]
- regulation of sensory perception of pain [ISO]
- response to bacterium [IMP]
- response to ethanol [ISO]
- response to fatty acid [ISO]
- response to lipopolysaccharide [IGI, IMP, ISO]
- response to oxidative stress [ISO]
- toll-like receptor signaling pathway [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The Tyrosine Kinase Syk Differentially Regulates Toll-like Receptor Signaling Downstream of the Adaptor Molecules TRAF6 and TRAF3.
Toll-like receptors (TLRs) are a major family of pattern recognition receptors, and they play a crucial role in innate immune responses. Activation of TLR4 signaling at the plasma membrane by its ligand lipopolysaccharide (LPS) stimulates a proinflammatory pathway dependent on the E3 ubiquitin ligase TRAF6 (tumor necrosis factor receptor-associated factor 6) and the kinase TAK1 (transforming growth factor β-activated kinase ... [more]
Sci Signal Aug. 22, 2013; 6(289);ra71 [Pubmed: 23962979]
Throughput
- Low Throughput
Ontology Terms
- raw-264.7 cell (BTO:0003292)
- macrophage (BTO:0000801)
Additional Notes
- incresed with LPS treatment
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TLR4 TRAF6 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID