An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Spatiotemporal coupling of cAMP transporter to CFTR chloride channel function in the gut epithelia.

Li C, Krishnamurthy PC, Penmatsa H, Marrs KL, Wang XQ, Zaccolo M, Jalink K, Li M, Nelson DJ, Schuetz JD, Naren AP

Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated chloride channel localized at apical cell membranes and exists in macromolecular complexes with a variety of signaling and transporter molecules. Here, we report that the multidrug resistance protein 4 (MRP4), a cAMP transporter, functionally and physically associates with CFTR. Adenosine-stimulated CFTR-mediated chloride currents are potentiated by MRP4 inhibition, and this potentiation ... [more]

Cell Nov. 30, 2007; 131(5);940-51 [Pubmed: 18045536]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SNTA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase binding [IPI]ion channel binding [IPI]nitric-oxide synthase binding [IPI]protein binding [IPI]sodium channel regulator activity [IMP]

Gene Ontology Cellular Component

ABCC4