Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Interaction between the triglyceride lipase ATGL and the Arf1 activator GBF1.

Ellong EN, Soni KG, Bui QT, Sougrat R, Golinelli-Cohen MP, Jackson CL

The Arf1 exchange factor GBF1 (Golgi Brefeldin A resistance factor 1) and its effector COPI are required for delivery of ATGL (adipose triglyceride lipase) to lipid droplets (LDs). Using yeast two hybrid, co-immunoprecipitation in mammalian cells and direct protein binding approaches, we report here that GBF1 and ATGL interact directly and in cells, through multiple contact sites on each protein. ... [more]

PLoS ONE Jul. 27, 2011; 6(7);e21889 [Pubmed: 21789191]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ARFGEF2 PNPLA2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Ellong EN (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ARFGEF2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ARF guanyl-nucleotide exchange factor activity [IBA, IDA]GABA receptor binding [ISS]guanyl-nucleotide exchange factor activity [IDA]protein binding [IPI]protein kinase A regulatory subunit binding [IDA]

Gene Ontology Cellular Component

PNPLA2