PNPLA2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PLIN5
Gene Ontology Biological Process
- lipid particle organization [IMP]
- lipid storage [IMP]
- mitochondrion localization [IMP]
- negative regulation of fatty acid beta-oxidation [IDA, IMP]
- negative regulation of lipase activity [IMP]
- negative regulation of lipid catabolic process [IDA]
- negative regulation of peroxisome proliferator activated receptor signaling pathway [IDA]
- negative regulation of reactive oxygen species metabolic process [IMP]
- negative regulation of triglyceride catabolic process [IDA]
- positive regulation of fatty acid beta-oxidation [IDA, ISO]
- positive regulation of lipase activity [ISO]
- positive regulation of lipid storage [ISO]
- positive regulation of sequestering of triglyceride [IDA, ISO]
- positive regulation of triglyceride biosynthetic process [IDA, IMP]
Gene Ontology Molecular Function
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
Unique regulation of adipose triglyceride lipase (ATGL) by perilipin 5, a lipid droplet-associated protein.
Lipolysis is a critical metabolic pathway contributing to energy homeostasis through degradation of triacylglycerides stored in lipid droplets (LDs), releasing fatty acids. Neutral lipid lipases act at the oil/water interface. In mammalian cells, LD surfaces are coated with one or more members of the perilipin protein family, which serve important functions in regulating lipolysis. We investigated mechanisms by which three ... [more]
Throughput
- Low Throughput
Additional Notes
- anisotropy forster resonance energy transfer
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PNPLA2 PLIN5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PLIN5 PNPLA2 | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | Low | - | BioGRID | 1527845 |
Curated By
- BioGRID