BAIT

PTGER3

EP3, EP3-I, EP3-II, EP3-III, EP3-IV, EP3e, PGE2-R, RP5-952N6.2
prostaglandin E receptor 3 (subtype EP3)
Homo sapiens
PREY

MKLN1

TWA2
muskelin 1, intracellular mediator containing kelch motifs
UPS Project
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Receptor isoform-specific interaction of prostaglandin EP3 receptor with muskelin.

Hasegawa H, Katoh H, Fujita H, Mori K, Negishi M

By using the yeast two-hybrid system, muskelin was found to bind with the carboxy-terminal tail of the prostaglandin EP3 receptor alpha isoform but not with either the beta or gamma isoform. A direct interaction between the carboxy-terminal tail of the alpha isoform and muskelin was confirmed in vitro using recombinant fusion proteins. Analysis by confocal microscopy indicated that the isoform ... [more]

Biochem. Biophys. Res. Commun. Sep. 16, 2000; 276(1);350-4 [Pubmed: 11006128]

Throughput

  • Low Throughput

Additional Notes

  • EP3 alpha C-terminal tail interacted with muskelin
  • Isoforms (Uniprot IDs): P43115-2 interacts with Q9UL63

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PTGER3 MKLN1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
904156

Curated By

  • BioGRID