BAIT

NEDD4L

NEDD4-2, NEDD4.2, RSP5, hNEDD4-2
neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
GO Process (33)
GO Function (7)
GO Component (7)

Gene Ontology Biological Process

Homo sapiens
PREY

UBE2N

HEL-S-71, UBC13, UBCHBEN; UBC13, UbcH-ben, UbcH13
ubiquitin-conjugating enzyme E2N
GO Process (30)
GO Function (7)
GO Component (8)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

OTUB1 enhances TGFβ signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3.

Herhaus L, Al-Salihi M, Macartney T, Weidlich S, Sapkota GP

SMAD transcription factors are key intracellular transducers of TGFβ cytokines. SMADs are tightly regulated to ensure balanced cellular responses to TGFβ signals. Ubiquitylation has a key role in regulating SMAD stability and activity. Several E3 ubiquitin ligases that regulate the turnover of SMADs are known; however, proteins that prevent the ubiquitylation or cause deubiquitylation of active SMADs remain undefined. Here ... [more]

Nat Commun Sep. 27, 2013; 4(0);2519 [Pubmed: 24071738]

Throughput

  • Low Throughput

Additional Notes

  • e1
  • e2- ube2d1, ube2n

Curated By

  • BioGRID