MPH1
Gene Ontology Biological Process
Gene Ontology Molecular Function
MUS81
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
Mph1 and mus81-mms4 prevent aberrant processing of mitotic recombination intermediates.
Homology-dependent repair of double-strand breaks (DSBs) from nonsister templates has the potential to generate loss of heterozygosity or genome rearrangements. Here we show that the Saccharomyces cerevisiae Mph1 helicase prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage. A role for Yen1 is only apparent in the absence of Mus81. Cells lacking Mph1 and the three ... [more]
Throughput
- Low Throughput
Ontology Terms
- chemical: methyl methanesulfonate (CHEBI:25255)
- phenotype: ionizing radiation resistance (APO:0000194)
- phenotype: resistance to chemicals (APO:0000087)
- phenotype: chromosome/plasmid maintenance (APO:0000143)
- phenotype: vegetative growth (APO:0000106)
Additional Notes
- double mutants show an increase in the in the accumulation of intersister JMs
- double mutants show decreased plating efficiency
- double mutants show increased sensitivity to ionizing rations and MMS
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MUS81 MPH1 | Dosage Lethality Dosage Lethality A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 1537666 | |
| MPH1 MUS81 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3519 | BioGRID | 2604885 | |
| MUS81 MPH1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | Low | -0.204 | BioGRID | 560527 | |
| MPH1 MUS81 | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 1537670 | |
| MPH1 MUS81 | Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores. | High | 0.1202 | BioGRID | 2605164 | |
| MUS81 MPH1 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 955738 | |
| MPH1 MUS81 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 353265 |
Curated By
- BioGRID