BAIT

ARRB1

ARB1, ARR1
arrestin, beta 1
GO Process (22)
GO Function (9)
GO Component (11)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Role of beta-arrestin 1 in the metastatic progression of colorectal cancer.

Buchanan FG, Gorden DL, Matta P, Shi Q, Matrisian LM, DuBois RN

G protein-coupled receptor ligand-dependent transactivation of growth factor receptors has been implicated in human cancer cell proliferation, migration, and cell survival. For example, prostaglandin E(2) (PGE(2))-induced transactivation of the EGF receptor (EGFR) in colorectal carcinoma cells is mediated by means of a c-Src-dependent mechanism and regulates cell proliferation and migration. Recent evidence indicates that beta-arrestin 1 may act as an ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jan. 31, 2006; 103(5);1492-7 [Pubmed: 16432186]

Throughput

  • Low Throughput

Ontology Terms

  • colonic adenocarcinoma cell line (BTO:0001913)
  • colon adenocarcinoma (DOID:234)
  • ls-174t cell (BTO:0001553)

Curated By

  • BioGRID