An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Protein kinase A-mediated phosphorylation of the Galpha13 switch I region alters the Galphabetagamma13-G protein-coupled receptor complex and inhibits Rho activation.

Manganello JM, Huang JS, Kozasa T, Voyno-Yasenetskaya TA, Le Breton GC

The present studies mapped the protein kinase A (PKA) phosphorylation site of Galpha(13) and studied the consequences of its phosphorylation. Initial experiments using purified human Galpha(13) and the PKA catalytic subunit established that PKA directly phosphorylates Galpha(13). The location of this phosphorylation site was next investigated with a new synthetic peptide (G(13)SRI(pep)) containing the PKA consensus sequence (Arg-Arg-Pro-Thr(203)) within the ... [more]

J. Biol. Chem. Jan. 03, 2003; 278(1);124-30 [Pubmed: 12399457]

Throughput

Low Throughput

Ontology Terms

8-bromo-3',5'-cyclic amp (CHEBI:64211)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TBXA2R GNA13	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Manganello JM (1999)	Low	-	BioGRID	908014
GNA13 TBXA2R	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Offermanns S (1994)	Low	-	BioGRID	306107
TBXA2R GNA13	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Djellas Y (1999)	Low	-	BioGRID	-
GNA13 TBXA2R	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Djellas Y (1999)	Low	-	BioGRID	-
TBXA2R GNA13	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Gales C (2005)	Low	-	BioGRID	721526

Curated By

BioGRID

Interaction Summary

TBXA2R

Gene Ontology Biological Process

Gene Ontology Molecular Function

guanyl-nucleotide exchange factor activity [TAS]protein binding [IPI]thromboxane A2 receptor activity [TAS]

Gene Ontology Cellular Component

GNA13

Gene Ontology Biological Process

Gene Ontology Molecular Function

D5 dopamine receptor binding [IBA]G-protein beta/gamma-subunit complex binding [IBA]GTPase activity [IBA]protein binding [IPI]signal transducer activity [IBA]type 1 angiotensin receptor binding [IBA]