BAIT

BUD32

LDB14, serine/threonine protein kinase BUD32, YGR262C
Protein kinase; component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; Pyrococcus Bud32 ortholog functions as a P-loop ATPase rather than a protein kinase in the context of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

HMO1

HSM2, L000003234, YDR174W
Chromatin associated high mobility group (HMG) family member; involved in compacting, bending, bridging and looping DNA; rDNA-binding component that regulates transcription from RNA polymerase I promoters; regulates start site selection of ribosomal protein genes via RNA polymerase II promoters; role in genome maintenance; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase; relocalizes to the cytosol in response to hypoxia
Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Functional wiring of the yeast kinome revealed by global analysis of genetic network motifs.

Sharifpoor S, van Dyk D, Costanzo M, Baryshnikova A, Friesen H, Douglas AC, Youn JY, Vandersluis B, Myers CL, Papp B, Boone C, Andrews BJ

A combinatorial genetic perturbation strategy was applied to interrogate the yeast kinome on a genome-wide scale. We assessed the global effects of gene overexpression or gene deletion to map an integrated genetic interaction network of synthetic dosage lethal (SDL) and loss-of-function genetic interactions (GIs) for 92 kinases, producing a meta-network of 8700 GIs enriched for pathways known to be regulated ... [more]

Unknown Feb. 17, 2012; 0(0); [Pubmed: 22282571]

Quantitative Score

  • -0.298 [SGA Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: colony size (APO:0000063)

Additional Notes

  • score threshold <= -0.12, interaction detected by Synthetic Genetic Array (SGA)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BUD32 HMO1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2978BioGRID
384464

Curated By

  • BioGRID