BAIT

CLA4

ERC10, serine/threonine protein kinase CLA4, L000000564, L000002643, YNL298W

Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication

Kinome Project (Sc)

GO Process (8)

GO Function (3)

GO Component (3)

Gene Ontology Biological Process

negative regulation of gene expression [IGI, IMP]

positive regulation of protein import into nucleus [IMP]

protein phosphorylation [IDA]

regulation of exit from mitosis [IMP]

response to pheromone [IGI]

septin ring organization [IGI, IMP]

sterol import [IMP]

vacuole inheritance [IGI, IMP]

Gene Ontology Molecular Function

kinase activity [IDA]
protein kinase activity [IDA]
protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

cellular bud [IDA]

fungal-type vacuole [IDA]

nucleus [IMP]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SWI4

ART1, SBF complex DNA-binding subunit SWI4, L000000124, L000002252, YER111C

DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p

GO Process (2)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

positive regulation of transcription from RNA polymerase II promoter in response to heat stress [IMP]

positive regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

SBF transcription complex [IDA, IMP, IPI]

nuclear chromatin [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Functional wiring of the yeast kinome revealed by global analysis of genetic network motifs.

Sharifpoor S, van Dyk D, Costanzo M, Baryshnikova A, Friesen H, Douglas AC, Youn JY, Vandersluis B, Myers CL, Papp B, Boone C, Andrews BJ

A combinatorial genetic perturbation strategy was applied to interrogate the yeast kinome on a genome-wide scale. We assessed the global effects of gene overexpression or gene deletion to map an integrated genetic interaction network of synthetic dosage lethal (SDL) and loss-of-function genetic interactions (GIs) for 92 kinases, producing a meta-network of 8700 GIs enriched for pathways known to be regulated ... [more]

Unknown Feb. 17, 2012; 0(0); [Pubmed: 22282571]

Quantitative Score

-0.284 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)
phenotype: colony size (APO:0000063)

Additional Notes

score threshold <= -0.12, interaction detected by Synthetic Genetic Array (SGA)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CLA4 SWI4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Bandyopadhyay S (2010)	High	-14.9021	BioGRID	541002
CLA4 SWI4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Collins SR (2007)	High	-17.9744	BioGRID	213393
CLA4 SWI4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.2838	BioGRID	407841
CLA4 SWI4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.3406	BioGRID	2175153
SWI4 CLA4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Fiedler D (2009)	High	-15.0929	BioGRID	325373
CLA4 SWI4	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Goehring AS (2003)	Low	-	BioGRID	80956
SWI4 CLA4	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Jorgensen P (2002)	High	-	BioGRID	165707
CLA4 SWI4	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tong AH (2004)	High	-	BioGRID	450152

Curated By

BioGRID

Interaction Summary

CLA4

Gene Ontology Biological Process

Gene Ontology Molecular Function

kinase activity [IDA]protein kinase activity [IDA]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

SWI4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

Negative Genetic

Publication

Functional wiring of the yeast kinome revealed by global analysis of genetic network motifs.

Quantitative Score

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

kinase activity [IDA]
protein kinase activity [IDA]
protein serine/threonine kinase activity [IDA]

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
sequence-specific DNA binding [IDA]