An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

USP47 and C terminus of Hsp70-interacting protein (CHIP) antagonistically regulate katanin-p60-mediated axonal growth.

Yang SW, Oh KH, Park E, Chang HM, Park JM, Seong MW, Ka SH, Song WK, Park DE, Baas PW, Jeon YJ, Chung CH

Katanin is a heterodimeric enzyme that severs and disassembles microtubules. While the p60 subunit has the enzyme activity, the p80 subunit regulates the p60 activity. The microtubule-severing activity of katanin plays an essential role in axonal growth. However, the mechanisms by which neuronal cells regulate the expression of katanin-p60 remains unknown. Here we showed that USP47 and C terminus of ... [more]

J. Neurosci. Jul. 31, 2013; 33(31);12728-38 [Pubmed: 23904609]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

KATNA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

microtubule binding [IDA]microtubule-severing ATPase activity [IDA]protein binding [IPI]protein heterodimerization activity [IPI]

Gene Ontology Cellular Component

HSP90AA1