BAIT

HECW2

NEDL2

HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2

GO Process (1)

GO Function (1)

GO Component (2)

Gene Ontology Biological Process

protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IBA]

Gene Ontology Molecular Function

ubiquitin-protein transferase activity [IBA]

Gene Ontology Cellular Component

cytoplasm [IBA]

CRISPR Database HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

PREY

SNW1

Bx42, NCOA-62, PRPF45, Prp45, SKIIP, SKIP

SNW domain containing 1

Cell Cycle - Mitotic Spindle Assembly Project

GO Process (19)

GO Function (9)

GO Component (7)

Gene Ontology Biological Process

Notch signaling pathway [TAS]

cellular response to retinoic acid [IDA]

gene expression [TAS]

intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]

mRNA splicing, via spliceosome [IC, IDA]

negative regulation of transcription from RNA polymerase II promoter [IDA]

negative regulation of transcription, DNA-templated [IDA]

positive regulation by host of viral transcription [IDA, IMP]

positive regulation of histone H3-K4 methylation [IMP]

positive regulation of mRNA splicing, via spliceosome [IMP]

positive regulation of neurogenesis [ISS]

positive regulation of transcription from RNA polymerase II promoter [IDA]

positive regulation of transforming growth factor beta receptor signaling pathway [IDA]

positive regulation of vitamin D receptor signaling pathway [IDA]

regulation of retinoic acid receptor signaling pathway [IDA]

regulation of transcription from RNA polymerase II promoter [TAS]

regulation of vitamin D receptor signaling pathway [IDA]

retinoic acid receptor signaling pathway [IDA]

transcription initiation from RNA polymerase II promoter [TAS]

Gene Ontology Molecular Function

Notch binding [IPI]
SMAD binding [IDA]
nuclear hormone receptor binding [IDA]
poly(A) RNA binding [IDA]
protein binding [IPI]
retinoic acid receptor binding [IDA]
transcription coactivator activity [IDA]
transcription corepressor activity [IDA]
vitamin D receptor binding [IDA]

Gene Ontology Cellular Component

SMAD3-SMAD4 protein complex [IDA]

catalytic step 2 spliceosome [IDA]

nuclear matrix [IDA]

nucleoplasm [IDA, TAS]

positive transcription elongation factor complex b [IDA]

spliceosomal complex [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

HECT Type Ubiquitin Ligase NEDL2 is Degraded by APC/C-Cdh1 and its Tight Regulation Maintains the Metaphase to Anaphase Transition.

Lu L, Hu S, Wei R, Qiu X, Lu K, Fu Y, Li H, Xing G, Li D, Peng R, He F, Zhang L

NEDD4-like ubiquitin ligase 2 (NEDL2) is a HECT type ubiquitin ligase. NEDL2 enhances p73 transcriptional activity and degrades ATR kinase in lamin misexpressed cells. Compared to the important functions of other HECT type ubiquitin ligase, there is less study concerning the function and regulation of NEDL2. Using primary antibody immunoprecipitation and mass spectrometry (IP/MS), we identify a list of potential ... [more]

J. Biol. Chem. Oct. 25, 2013; 0(0); [Pubmed: 24163370]

Throughput

High Throughput

Curated By

BioGRID