BAIT

RPB7

DNA-directed RNA polymerase II subunit RPB7, B16, L000001681, YDR404C

RNA polymerase II subunit B16; forms dissociable heterodimer with Rpb4p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation

GO Process (5)

GO Function (4)

GO Component (4)

Gene Ontology Biological Process

nuclear-transcribed mRNA catabolic process, exonucleolytic [IGI]

positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [IMP]

positive regulation of translational initiation [IMP]

transcription from RNA polymerase II promoter [IGI]

transcription initiation from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

RNA-directed RNA polymerase activity [IDA]
single-stranded DNA binding [IDA, IMP]
single-stranded RNA binding [IDA, IMP]
translation initiation factor binding [IPI]

Gene Ontology Cellular Component

DNA-directed RNA polymerase II, core complex [IDA]

cytoplasm [IDA]

cytoplasmic mRNA processing body [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

DGR2

YKL121W

Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds; DGR2 has a paralog, YMR102C, that arose from the whole genome duplication

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The RNA polymerase II Rpb4/7 subcomplex regulates cellular lifespan through an mRNA decay process.

Duan R, Rhie BH, Ryu HY, Ahn SH

In budding yeast, a highly conserved heterodimeric protein complex that is composed of the Rpb4 and Rpb7 proteins within RNA polymerase II shuttles between the nucleus and cytoplasm where it coordinates various steps of gene expression by associating with mRNAs. Although distinct stages of gene expression potentially contribute to the regulation of cellular lifespan, little is known about the underlying ... [more]

Biochem. Biophys. Res. Commun. Nov. 08, 2013; 441(1);266-70 [Pubmed: 24358479]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPB7 DGR2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Duan R (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary