BAIT

CKS2

CKSHS2, RP11-8C21.2
CDC28 protein kinase regulatory subunit 2
GO Process (2)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Homo sapiens
PREY

LDHB

HEL-S-281, LDH-B, LDH-H, LDHBD, TRG-5
lactate dehydrogenase B
GO Process (3)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

CKS proteins protect mitochondrial genome integrity by interacting with mitochondrial single-stranded DNA-binding protein.

Radulovic M, Crane E, Crawford M, Godovac-Zimmermann J, Yu VP

Cyclin-dependent kinase subunit (CKS) proteins interact with cyclin-dependent kinases (CDKs) with high affinity. Mammalian CKS1 and CKS2 bind CDK1 and CDK2 and partake in the control of cell cycle progression. We identified CKS-interacting proteins by affinity purification followed by mass spectrometry in the human lymphocytic cell line Ramos. Apart from known interactors, such as CDKs, we identified a novel CDK-dependent ... [more]

Mol. Cell Proteomics Jan. 01, 2010; 9(1);145-52 [Pubmed: 19786724]

Throughput

  • Low Throughput

Curated By

  • BioGRID